John Greenman UOH_3982a (1)

Professor John Greenman

Professor of Tumour Immunology

Faculty of Health Sciences

School of Life Sciences

j.greenman@hull.ac.uk

01482 465502

John is a tumour immunologist with extensive experience of developing lab on a chip technology for analysing tumour biopsies.

He has published over 150 peer-reviewed publications and his immunobiology group works closely with clinicians, chemists and engineers.

The majority of his research work has focused on tumours of the head and neck region, identifying novel markers of progression or treatment response.

Research

Research Interests

Coming from an immunology background, Professor Greenman is extremely interested in understanding how the immune system is often subverted during carcinogenesis, and more importantly how such knowledge can be used clinically (diagnostically, prognostically or therapeutically).

Research groups

Current and recent grants

H2020-MSCA-RISE-2016, €976,000 (€153k to Hull) (Greenman). Blood test for clinical therapy guidance of non-small cell lung cancer patients.

Yorkshire Cancer Research, £99,198 (Lind and Greenman). Peripheral blood detection of EGFR status in lung cancer patients. 01/11/2016 – 31/10/2018

Innovate UK/EPSRC £496,000 (£193k to Hull) (Greenman, Green, Cawthorne). Development of a multi-purpose small animal phantom for pre-clinical radiotherapy studies. 01/03/2016 – 28/02/2018.

Yorkshire Cancer Research £123,312 (Archibald, Madden & Greenman). Chemokine receptor targeted therapies. 01/02/2014 – 31/01/2016.

British Lung Foundation £24,800 (Loveday, Lind, Haswell & Greenman). Development of microfluidic device for response to chemotherapy in malignant mesothelioma. 01/10/2013 – 30/09/2015.

Technology Strategy Board Sepsis I: Multi-pathogen detection and/or simple discrimination £399,000 (Greenman, Dyer, Haswell, BioGene Ltd). Rapid, on-chip, multiplexed detection of sepsis-causing organisms from blood samples. 01/09/2012 – 31/08/2015.

FP7-SME-2012. Point-of-care blood device for fast and reliable prediction of drug response in non-small cell lung carcinoma patients from blood samples (LungCARD 315586) University of Hull (Haswell & Greenman); Hull and East Riding NHS Trust (Lind & Greenman) (£306,000 to Hull; 1.2M Euro total) 01/01/2013 – 28/2/2014.

NC3R £450,000 (Greenman, Madden, Flynn, Haswell, Maraveyas). Replacement of animal models for tumour biology with a multifunctional microfluidic-based approach. 01/10/2011 – 30/09/2014.

NIHR (i4i programme). £667,542 (Elliott, Wadhawan, Singh, MacFie, Greenman). Development of a placement sensing nasogastric tube. 01/07/2011 – 30/06/2014.

Hull and East Riding Cardiac Trust. £76,128 (Greenman, Jones, Dyer, Wadhawan, Haswell, Griffin, Seymour) Integrated microfluidic devices for real-time measurement of biochemical changes in heart tissue. 01/03/2011 – 30/11/2012.

Heart Research UK £199,728 (Greenman, Haswell and Seymour). Development of microfluidic devices for analysis of function in normal and diseased cardiac tissue. 10/2007 – 09/2011.

EPSRC EP/H007385 £210,949 (Haswell & Greenman). Commercialisation of lab-on-a-chip technology for DNA profiling. 08/2009 – 07/2010.

Publications

1. Dawson, A., Dyer, CE., Macfie, J., Davies, J., Karsai, L., Greenman, J., Jacobsen, M. (2016), 'A Microfluidic Chip Based Model for the Study of Full Thickness Human Intestinal Tissue Using Dual Flow', Biomicrofluidics, 10, 064101; doi.org/10.1063/1.4964813

2. Ettelaie, C., Collier, ME., Featherby, S., Benelhaj, NE., Greenman, J., Maraveyas, A. (2016), 'Analysis of the Potential of Cancer Cell Lines to Release Tissue Factor-Containing Microvesicles: Correlation with Tissue Factor and PAR2 Expression', Thrombosis Journal BBA - Molecular Cell Research, 1863(11) 2846-2857.

3. Wong, BYW, Stafford, ND., Green, VL., Greenman, J. (2016), 'Prognostic Value of the Neutrophil to Lymphocyte Ratio in Lryngeal Squamous Cell Carcinoma Patients', Head & Neck, 38 E1903-8.

4. Phurimsak, C., Tarn, M., Peyman, S., Greenman, J, Pamme, N (2014), 'On-Chip Determination of C-Reactive Protein Using Magnetic Particles in Continuous Flow', Analytical Chemistry, 86 (21) 10552-9.

5. Drennan, S., Stafford, ND., Greenman, J., Green, VL. (2014), 'Frequency Over Function: Raised Levels of CD127low/- Regulatory T Cells in the Tumour Microenvironment Compared with the Periphery of Head and Neck Cancer Patients', Journal of Clinical & Cellular Immunology, 5:241. doi 10.4172/2155-9899.1000241

6. Drennan, S., Stafford, ND., Greenman, J., Green, VL. (2013), 'Increased Frequency and Suppressive Activity of Cd127low/- Tregs in the Peripheral Circulation of Patients with Head and Neck Squamous Cell Carcinoma are Associated with Advanced Stage and Nodal Involvement', Immunology, 140 (3) 335-343.

Teaching

Programmes taught on

Modules list

BSc programmes

  • Clinical Microbiology and Immunology

  • Professional and Research skills for Biomedical Sciences

  • Reviews in Biomedical Science

  • Final Year Research Projects, 20 and 40 credit

MSc programmes
  • Tumour Immunology and Microenvironment

  • Organ-specific Cancers: From Bench to Bedside

  • Research Project and Dissertation

Research PhDs

I welcome applications to work with me in either the biomedical applications of microfluidic devices or head and neck immunobiology. These can come from basic scientists or medically trained individuals.

Completed PhDs

60 postgraduate (PhD and MD) students successfully supervised since 1999.

PhD
Cheah, R. (2016), Monitoring the Response of Head and Neck Tumour Tissue to Irradiation Using a Microfluidic-Based Approach

Smith, J. (2016), The Effect of HNSCC-derived Soluble Factors on the Proliferation and Function of Immune Cells

Chandrabalan, K. (2016), A Microfluidic Approach to the Study of Cellular Responses to Oxidative Stress

Williamson, J. (2016), The Role of Endothelial Adhesins in Leukocyte Adhesion in Response to Pharmaceutical Agents that Induce Pulmonary Fibrosis

Todd, A. (2016), Role of Hypoxia-Induced Adam 10 in Colorectal Cancer Progression

Pacelli, A. (2015), Development of a Pet Probe for the Imaging of Cox-2 Expression in Cancers


MD
Patel, R. (2016), An Observational Pilot Study to Assess the Potential of a Microfluidic Tissue Culture Model to Predict Rectal Cancer Response to Neo-Adjuvant Therapy

Zahoor, T. (2016), Does HPV-16 Seropositivity Correlate with T-cell Distribution Providing Additional Prognostic Information in Infected HNSCC Patients?

Carr, S. (2013), Assessing the Effects of Radiotherapy on Head and Neck Squamous Cell Carcinoma using Microfluidic Techniques 

Current PhD supervisors

Currently acting as primary and secondary supervisor to 18 postgraduate students covering all aspects of my research interests (three are currently writing up).

Teaching awards and accolades

External examiner:

  • University of Keele Biomedical Science (BSc),(2012-16)

  • Imperial College, MBBS (Year 2) (2002-05)

Professional highlights

Academic qualifications

  • BSc (Hons) Pathobiology (with Chemistry) (1987) University of Reading

  • Doctor of Philosophy (Immunochemistry), (1991) University of Southampton

  • 1990-1991 Post-doctoral Research Fellow, Tenovus Cancer Research Unit, University of Southampton

External roles

Recent invitations

3rd International Conference on Herbal and Synthetic Drug Studies (HSD-2016), Pune, India HSD2016.com), 7-9 January 2016, 'Use of Microfluidic Devices for Drug Testing Tissue Biopsies'.

SelectBio Organ-on-a-Chip Europe, Cambridge UK, 5-6 April 2016, 'Microfluidic Chips and Cancer Treatment'.

Precision Cancer Medicine: Forefront Technologies at the Clinical Interface, Southampton, UK, 14 September 2017, 'Tissue-based Microfluidic Devices for Personalised Treatment of Patients with Cancer'.

Awards and accolades

Heart Research UK’s Researcher of the Year in 2011

Professional highlights

1991–1995 Post-doctoral Research Fellow, Sir William Dunn School of Pathology, MRC Cellular Immunology Unit, University of Oxford

1995-1999 Lecturer in Immunology, Dept of Surgery, University of Hull

1999-2004 Senior Lecturer in Tumour Immunology, University of Hull

2004- 2009 Reader in Tumour Immunology, University of Hull

Currently a member of the NC3Rs David Sainsbury Fellowship Panel 2015 -

REF2014 - Appointed as Output Assessor for Panel UoA3 (2014)